Publications
CASE STUDY: Myeloid Progenitor Cell Differentiation
Screening for Compounds that Induce Myeloid Progenitor Cell Differentiation Using the HyperCyt® High Capacity Flow (HCF) Screening Platform, Thomas D. Duensing and David B. Sykes
Publications
Biologics Discovery
POSTER: A Novel, No Wash Screening Assay to Simultaneously Measure Binding to Target and Control Antigens on Mammalian Cell Surfaces. Yen Kim Luu, Zhaoping Liu, Mara Abashian and Thomas D. Duensing. Antibody Engineering 2011. Download Poster
POSTER: Development of a High Throughput Screening Assay that Simultaneously Quantifies Antibody Levels, Cell Number and Cell Viability. Thomas D. Duensing, Zhaoping Liu, Mara Abashian and Yen Kim Luu. Antibody Engineering 2010. Download Poster
POSTER: Development Of A Multiplex High Throughput Screening Assay Cascade For Evaluating Receptor Tyrosine Kinase Binding And Activation. Linda Trinkle, Thomas D. Duensing, Zhaoping Liu, Mara Abashian and Yen Kim Luu. Antibody Engineering 2011. Download Poster
APPLICATION BRIEF: A Mix-and-Measure Assay for Screening Hybridoma Supernatants on IntelliCyt Platforms.
POSTER: Development of a No Wash Bead-based High Throughput IgG Titre Assay; Joanne Yetz-Aldape, Tianxiao Sun, David Choiniere, Thomas Duensing, Linda Trinkle, Geoffrey Hodge. ISAC CYTO2010
POSTER: A Novel Method to Simultaneously Quantify Antibody Production and Cell Health Utilizing the HyperCyt System for Cell-based Screening; Thomas Duensing, Mara Abashian, Barbara Stout and Christopher Black. SBS Annual Meeting (2010)
Cell-based Screening
REVIEW: Cell-Based Screening Using High-Throughput Flow Cytometry; Christopher B. Black, Thomas D. Duensing, Linda S. Trinkle, and R. Terry Dunlay, ASSAY and Drug Development Technologies.
High throughput flow cytometry for discovery at UNMCMD and the NIH Molecular Libraries Initiative; Larry A. Sklar and Bruce S. Edwards, Drug Discovery World, Fall 2010
Flow Cytometry for Drug Discovery, Receptor Pharmacology and High-Throughput Screening; Larry A Sklar, Mark B. Carter, and Bruce S. Edwards. Current Opinion Pharmacology, 2007.
Poster: HyperCytPRO: An Advanced Platform for Collecting, Managing and Sharing Data from Multiplate Flow Cytometry Screening Experiments; Linda Trinkle, Aaron Kennington, Thomas Duensing. SBS Annual Meeting (2010)
Use of Flow Cytometric Methods to Quantify Protein: Protein Interactions; Levi L. Blazer1, David L. Roman2, Molly R . Muxlow1, Richard R. Neubig, Current Protocols in Cytometry UNIT 13.11 Online Posting Date: January, 2010
In Vitro Toxicology
Poster: A Multiplexed Screening Method Correlating Compound Toxicity Dose Effects with Cell Type, Cell Health and Culture conditions Utilizing the HyperCyt System for High throughput Flow Cytometry; Christopher Black, Barbara Stout, Mara Abashian and Thomas Duensing. SBS Annual Meeting (2010)
Small Molecule Discovery
Identification of a small GTPase inhibitor using a high-throughput flow cytometry bead-based multiplex assay; Surviladze Z, Waller A, Wu Y, Romero E, Edwards BS, Wandinger-Ness A, Sklar LA. J Biomol Screen., 2010 Jan;15(1):10-20. Epub 2009 Dec 11
Detection of Intracellular Granularity Induction in Prostate Cancer Cell Lines by Small Molecules Using the HyperCyt® High-Throughput Flow Cytometry System; Mark K. Haynes, J. Jacob Strouse, Anna Waller, Andrei Leitao, Ramona F. Curpan, Cristian Bologa, Tudor I. Oprea, Eric R. Prossnitz, Bruce S. Edwards, Larry A. Sklar, Todd A. Thompson; Journal of Biomolecular Screening. 2009:596-609
High-Throughput Flow Cytometry to Detect Selective Inhibitors of ABCB1, ABCC1, and ABCG2 Transporters; Irena Ivnitski-Steele, Richard S. Larson, Debbie M. Lovato, Hadya M. Khawaja, Stuart S. Winter, Tudor I. Oprea, Larry A. Sklar, Bruce S. Edwards. Assay and Drug Development Technologies. April 1, 2008, 6(2): 263-276. 2007.
High Throughput Flow Cytometric Analysis With HyperCyt®: Utilization of a 384 Well Microplate-Based Assay for the Detection of Ligand Binding to Cellular Receptors; Linda S. Trinkle, Bruce S. Edwards, Susan M. Young, Irena Ivnitsky-Steele, Richard D. Ye, Eric R. Prossnitz, Larry A. Sklar. Poster, Society for Biomolecular Sciences (SBS) 2007