HTFC^® FAQs

Is this a flow cytometer?

The HTFC system is an integrated state-of-the-art, 2-laser, 4-color flow cytometer with microplate sampler.

How many parameters can you collect?

You can collect 6 parameters—4 fluorescence plus 2 light scatter measurements.

How do I set the voltage gains?

The HTFC Cytometer has a broad dynamic range and does not require the user to manipulate voltage gain settings. All available sample data can be collected with excellent resolution using the factory settings. This feature eliminates a time consuming step in data collection, and greatly reduces the variability between users.

What is the minimum sample requirement per well?

Depending upon the microplate, specimens as small as 10 µl can be analyzed, with the actual uptake as low as 1-2 µl. Since there is no dead volume, the remaining specimen can be re-sampled for kinetics studies or further analysis.

Can I change the optical filters in the HTFC Cytometer?

Yes. The protocol for replacing optical filters is easy and takes less than 5 minutes.

Can I use the FL4 fluorescent detector with the blue laser?

No. FL4 is dedicated to the red laser.

Can I sort cells with HTFC Cytometer?

No. The HTFC Cytometer is an analytical cytometer. The test sample (cells) is discharged into the waste tank after analysis.

Can I use my current data analysis software to report results?

Yes. HyperView Data Analysis Software can take the plate data file and generate individual FCS data files for each well and export them to your current analysis program.

How much carryover can I expect to see between wells?

Carryover is sample dependent. 1-2% particle carryover has been observed in very small (<2 µl samples). Inter-well washes or larger samples decrease that to <<1%.

What kind of plates can you run?

The HTFC and HyperCyt can both handle a wide variety of 96- and 384-well plates. They will collect from both shallow and deep well plates and also bullet tubes in 96-well format.

Can you run 1536-well plates?

Currently the HTFC and HyperCyt do not support 1536-well format.

Can the HTFC work with a robotic plate loader?

The HTFC has an optional API that will enable communication with a robotic plate loader.